Can consuming the pre-workout supplement NO-Shotgun increase muscle gains?


Stimulating the synthesis of new muscle protein is the most important part of the muscle building process and heavy resistance training is the main way to do this. Not only will resistance training increase muscular size but it will also increase muscle strength and hypertrophy.

Resistance training alone will show results but there are ways of speeding up and enhancing muscle building. When speaking with our customers, there is an overwhelming demand for a supplement that aids the training session itself so we often recommend a pre-workout supplement that boosts energy levels, creates focus for the workout and supports muscle growth through providing protein peptides for maximum protein synthesis, development of muscle tissue and muscle repair.

This article investigates whether the consumption of the pre-workout supplement VPX NO Shotgun, when combined with resistance training, will boost muscle growth and strength, without negative side effects.


No Shotgun


A study by Shelmadine et al explores the effect that NO Shotgun has on muscle gains through clinical testing. To research this, 18 healthy, non-resistance trained males were chosen as the sample. Participants took part in a resistance training program 4 times/week over the course of 28 days. The sample was split into two groups, one group was given the placebo and the other ingested NO Shotgun 30 mins before exercise.

Various base rate values for each participant were determined before the exercise began and before either the NO Shotgun or the placebo was administered. Initial assessments included strength, body composition, blood sampling and muscle biopsies. These values were determined again after the 28 day study period to assess changes, if any.



Body mass increased in both samples, however, fat mass had no significant changes and fat-free mass increased in both samples, with the NO sample receiving greater gains in this. The NO Shotgun group experienced greater increases in bench press strength than the placebo group. All of the myogenic regulatory factors were increased with training. NO Shotgun was shown to be significantly greater than the placebo for muscle building proteins such as Myo-D and MRF-4.

Myofibrillar protein also increased with training and the group consuming NO Shotgun showed a significantly greater increase.  Total DNA was increased in both groups, and again, NO Shotgun was significantly more than placebo. Muscle Growth serum IGF-1 and HGF levels were significantly increased with training and for the NO Shotgun group HGF levels were greater than the placebo group.  No significant differences were located for any of the whole blood and serum clinical chemistry markers.


Side Effects

During the study period side effects were reported by both sample groups. Four of the participants who ingested the NO Shotgun reported side effects of dizziness, nausea, headache, rapid heart rate and nervousness. Four of the placebo sample also reported feelings of nausea, rapid heart rate and shortness of breath.


Our Comments

This study on the effectiveness of NO Shotgun has indicated that muscle gains will still occur through exercise alone and that muscle protein synthesis is increased without ingesting a pre-workout supplement, which of course, we already knew.

However, this study does support past research supporting the use of stimulants, as well as ergogenics such as creatine and beta alanine, as being effective means to boost muscle mass and training performance. With NO Shotgun being a proprietary blend it is difficult to isolate the effects of the various nutrients which comprise the product blend, but the inclusion of proven compounds such as Peptopro, creatine, and stimulants such as caffeine is undoubtedly the main reason behind the greater results seen in this study from the subjects consuming NO Shotgun.


Further Research

Longer term study would be useful to assess the on-going effects of NO Shotgun on muscle gains as the effects of any PWO are more noticeable in the first month. Ingesting a PWO 4 times a week is likely to give very noticeable effects, a study spanning a larger number of months on less frequent dosage would reveal the on-going usefulness of NO Shotgun. The comparison of a range of pre-workouts and their effects is also an option for further research.


Recommended Product

VPX NO Shotgun + FREE Predator Shaker 588g


Author: Rosie Smith



Shelmadine et al. (2009). Effects of 28 days of resistance exercise and consuming a commercially available pre-workout supplement, NO-Shotgun®, on body composition, muscle strength and mass, markers of satellite cell activation. Journal of the International Society of Sports Nutrition. 6 (16), 1550-2783.

© 2012, predatornutrition. All rights reserved.

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